Pseudomonas aeruginosa is one of the most common nosocomial pathogen causing opportunistic infections in humans, particularly among immuno compromised patients, and because of its ubiquitous nature, ability to survive in adverse conditions and affinity for moist environment remains a common pathogen in intensive care units (ICU). In the present study, prevalence rate of P. aeruginosa in maternity wards and labor rooms in various hospitals of Gulbarga region, South India and their antibiotic sensitivity pattern (AST) are reported. Present study was based on the 190 samples collected from animate and inanimate objects from maternity wards and labor rooms of several hospitals in Gulbarga region, during Oct 2008 to Jan 2010. A total of 43 P. aeruginosa were isolated, indicating on isolation rate of 22.63% and the isolation rate was slightly higher in maternity wards than the labor rooms. There is an increased multidrug resistance among P. aeruginosa which may be due to the selective pressure from the use of antimicrobial agents which is a major determinant for the convergence of resistant strains, especially in hospital environments.