Arbuscular mycorrhizal fungal database, named iAMF (www.amfungi.aurobindo.du.ac.in) is first digital database on biodiversity and phylogeny of these beneficial fungi in India. It provides access to data on 161 species of arbuscular mycorrhizal fungi (AMF) along with comprehensive information on their GPS location of reported occurrence, taxonomy, molecular sequence, phylogeny and bibliography. The database is based on primary data collected by authors through ongoing surveys (year 2009-2017) and secondary data from earlier studies (2008–2016). Data is carefully curated to verify that it corresponds to the reference publication and a valid species name is used. Apart from being ecological meta-analysis data source, it would have significant application in selection of non-indigenous AMF inoculum in agriculture, reforestation, horticultural and land reclamation practices.