The eukaryotes sterol pathways are extremely conserved and these biosynthetic pathway are very long which includes the synthesis of dolichols, coenzyme Q, heme A, and isoprenylated proteins.14-Demethylase is an essential enzyme of the cytochrome P450 superfamily, which is potential the target of azole antifungals. Predicted results shows that 14-alpha sterol demethylase have molecular weight of 58930.8 Daltons and the theoretical isoelectric point (pI) of 7.64. The negative Grand average of hydropathicity (GRAVY) index of ‐0.125. The Aliphatic index of Aspergillus fumigates 14-alpha sterol demethylase is 89.48. Alpha helix (Hh) accounts 210 amino acids of about 40.08%. The extended strand (Ee) had 91 amino acids accounting 17.37, Beta turn (Tt) made up of 51 amino acids making up 9.73% and random coil (Cc) made up of 172 amino acids accounting 32.82%. The subcellular localization of 14alpha sterol demethylase Cyp51B was predicted to be a Plasma membrane protein.
OBJECTIVES – Objective of this study is to evaluate the new bone formation in bony defects after placement of PRP and its efficacy of regenerative potential .
METHOD – Twenty five pediatric patients were selected for the study and PRP was extracted from patients own blood and placed in defect in all patients. Post operative clinical and radiographic observation was done at 1st,4th and 6 th month.
RESULTS – Faster bone healing in all patients was observed.
CONCLUSION – It can be concluded by the study that PRP is a better source of bone induction growth factors and regeneration of bone can be done at faster rate which is a favorable biological response similar to natural healing process